17552 Yy1 Status: Cancelled
| Gene | Yy1 |
| Chromosome | 12 |
| Deletion Start | 108,815,687 |
| Deletion End | 108,793,416 |
| Deletion Size | 22,272 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,794,022 | 108,794,042 | AAGTGGGAGCAGAAGCAGGTG |
| TUP | 108,794,043 | 108,794,067 | CAGATCAAGACCCTGGAGGGCGAGT |
| TUR | 108,794,073 | 108,794,091 | CCGAGGACCACATGGTGAC |
| TDF | 108,814,884 | 108,814,904 | GAAGGTCTGCTTGGGTTCCTG |
| TDP | 108,814,908 | 108,814,936 | ACTTACTGCATGTGACTGCAGGTGTTACA |
| TDR | 108,814,945 | 108,814,966 | TCTCCCGACAGATACTCCCATC |
PCR Primers
| Primer | Sequence |
| SU | TATTTGTGTGGAAGGAGGCG |
| SD | CGCAAGCAAAGTTATCAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
