17553 Zap70 Status: Cancelled
| Gene | Zap70 |
| Chromosome | 1 |
| Deletion Start | 36,782,615 |
| Deletion End | 36,770,814 |
| Deletion Size | 11,802 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,770,944 | 36,770,963 | GCTACGTGCTGTCGTTGGTG |
| TUP | 36,770,966 | 36,770,988 | CGACGTGCGCTTCCACCATTTCC |
| TUR | 36,770,989 | 36,771,006 | TGAGTTGGCGCTCGATGG |
| TDF | 36,781,876 | 36,781,899 | CCAGACCAGATGACAAGGAGATAC |
| TDP | 36,781,900 | 36,781,924 | CCAAACAAGCTGCCCTGTTCCAACA |
| TDR | 36,781,938 | 36,781,955 | CAGCGCCGTGGTTGTTTC |
PCR Primers
| Primer | Sequence |
| SU | ACACTATTGACCGCCACCTC |
| SD | ACTGGTTCCACTCAATTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 63 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
