17560 Zfp658 Status: Cancelled
| Gene | Zfp658 |
| Chromosome | 7 |
| Deletion Start | 43,575,006 |
| Deletion End | 43,564,787 |
| Deletion Size | 10,220 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,564,921 | 43,564,941 | CAGCTAAGAGCAGAGCACAGG |
| TUP | 43,564,945 | 43,564,972 | CAGTGCTCCTGTACCTCCTAGTCTGTCA |
| TUR | 43,564,980 | 43,564,999 | CACCAGGAGGCAGGACAATG |
| TDF | 43,574,573 | 43,574,592 | GGCTTCAAAGGGCCAACTTC |
| TDP | 43,574,597 | 43,574,620 | TCATCAGCGGATTCACACTGGCGA |
| TDR | 43,574,657 | 43,574,676 | GCAAAGACGACCTGCCAAAG |
PCR Primers
| Primer | Sequence |
| SU | TGGTCAATACCAGACAGGGC |
| SD | CATTCTCTTCAAGTAATTTAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
