17569 1190003J15Rik Status: Targeting vector QC completed
| Gene | 1190003J15Rik |
| Chromosome | 7 |
| Deletion Start | 148,021,523 |
| Deletion End | 148,023,706 |
| Deletion Size | 2,184 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 148,021,644 | 148,021,667 | GGACAAGGTAGGAGACAGTTTCTG |
| TUP | 148,021,671 | 148,021,694 | TGGGATCCATAAGTGCCAGCACCC |
| TUR | 148,021,746 | 148,021,765 | GCCTTACTGCTCCACCTCAG |
| TDF | 148,022,857 | 148,022,874 | TCCAGACCAGGGACAGAC |
| TDP | 148,022,881 | 148,022,903 | CACCTCTCCCAGGGTGGTAGCAA |
| TDR | 148,022,939 | 148,022,958 | TGCCTCAGGTTCCTGAACAC |
PCR Primers
| Primer | Sequence |
| SU | TGATGACGCTCCAGAGACAC |
| SD | CAATGATAGGTTCAGATTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 122 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
