| Gene | Brpf1 |
| Chromosome | 6 |
| Deletion Start | 113,324,198 |
| Deletion End | 113,309,816 |
| Deletion Size | 14,383 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,310,064 | 113,310,084 | ACCAGGCCGAGAGGTGATGAG |
| TUP | 113,310,086 | 113,310,106 | TATGCTCAGGCCCAGCGCATG |
| TUR | 113,310,107 | 113,310,128 | GGCCATGAAGGTCCACTTCTAC |
| TDF | 113,324,076 | 113,324,098 | CAAGTCAGTGCAGATTGCTTACC |
| TDP | 113,324,099 | 113,324,121 | ACAGGGCTCTGCAGCACCGAAGC |
| TDR | 113,324,165 | 113,324,184 | CACCGGGCTCTGAGAGTATC |
PCR Primers
| Primer | Sequence |
| SU | AAGGAAGACTAGGGACAGAGAGC |
| SD | CAGGCCTCTAGGCAGGTATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17579A-A1 | VGB6 | C57BL/6NTac | |
| 17579A-A5 | VGB6 | C57BL/6NTac | |
| 17579A-D6 | VGB6 | C57BL/6NTac | |
| 17579A-G4 | VGB6 | C57BL/6NTac | |
| 17579A-F4 | VGB6 | C57BL/6NTac | |
| 17579A-F7 | VGB6 | C57BL/6NTac | |
