17592 Cpa2 Status: Cancelled
| Gene | Cpa2 |
| Chromosome | 6 |
| Deletion Start | 30,564,469 |
| Deletion End | 30,541,668 |
| Deletion Size | 22,802 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,542,384 | 30,542,404 | CCCTCCAGTGGTCAGAATGTG |
| TUP | 30,542,408 | 30,542,434 | ATGACAGACAGCAGGGAAGACAGGCTA |
| TUR | 30,542,441 | 30,542,458 | CAACGCCTGAGGCAAGTG |
| TDF | 30,563,623 | 30,563,646 | AGCCTCCCTGGTCTCTGTTCTTAG |
| TDP | 30,563,649 | 30,563,674 | ACCTGGTAACTTCCTCCAACCTGTCC |
| TDR | 30,563,689 | 30,563,709 | AAACAATGGACTGGCCCTGAG |
PCR Primers
| Primer | Sequence |
| SU | AACAACTGATTTGCCCTTGG |
| SD | CAAGAGCTGGGTGTGGTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
