| Gene | Exosc8 |
| Chromosome | 3 |
| Deletion Start | 54,735,021 |
| Deletion End | 54,728,744 |
| Deletion Size | 6,278 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 54,734,595 | 54,734,614 | CGCGAAGTCACGCTAAGGTC |
| TUP | 54,734,569 | 54,734,594 | CCTGCTTCAGAGCACGAGTGATGTGA |
| TUR | 54,734,537 | 54,734,557 | CACACCCAGAAGAGGGTCAAC |
| TDF | 54,728,867 | 54,728,885 | GGGCTGACTGGAGCTAAAC |
| TDP | 54,728,839 | 54,728,864 | CAGGACTGCATGAGTCGAGCGGTAAC |
| TDR | 54,728,816 | 54,728,838 | GCTTGCTCACTTCTTTGTGTCTC |
PCR Primers
| Primer | Sequence |
| SU | ACCTGTGCACTGGCTTCAC |
| SD | AGAGGCATGCCAACTACTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17610A-C12 | VGB6 | C57BL/6NTac | |
| 17610A-F1 | VGB6 | C57BL/6NTac | |
| 17610A-F8 | VGB6 | C57BL/6NTac | |
| 17610A-F9 | VGB6 | C57BL/6NTac | |
| 17610A-F11 | VGB6 | C57BL/6NTac | |
| 17610A-B8 | VGB6 | C57BL/6NTac | |
