17619 Gabarapl2 Status: Cancelled
| Gene | Gabarapl2 |
| Chromosome | 8 |
| Deletion Start | 111,952,428 |
| Deletion End | 111,940,822 |
| Deletion Size | 11,607 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 111,941,453 | 111,941,473 | TCGGCTCCCTCACCAATTATG |
| TUP | 111,941,479 | 111,941,505 | AACCGTGTTCTAGGACAGGGCAACAGG |
| TUR | 111,941,530 | 111,941,550 | CAAATGCCAGTCTCCCTCCAC |
| TDF | 111,952,083 | 111,952,104 | TGTGCCTGCTAGGTTAATGTTG |
| TDP | 111,952,105 | 111,952,124 | TCAGTGAGCCACCGCCACCC |
| TDR | 111,952,172 | 111,952,192 | CCTGCTGTGACCTGCAATCTG |
PCR Primers
| Primer | Sequence |
| SU | AGTACTCGTTGCACACAGCG |
| SD | CAACATGATTTGCAGTTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
