17620 Galm Status: Cancelled
| Gene | Galm |
| Chromosome | 17 |
| Deletion Start | 80,150,163 |
| Deletion End | 80,127,719 |
| Deletion Size | 22,445 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,128,617 | 80,128,635 | GGGCCTCGTTCTGCCTAAG |
| TUP | 80,128,636 | 80,128,658 | CTGTCCTGGAGCAAGAGCCAGCC |
| TUR | 80,128,659 | 80,128,682 | CCTATGCGTGGATCAAGATGAAAC |
| TDF | 80,149,222 | 80,149,243 | CTGTTGCATGACAGGGAGTATG |
| TDP | 80,149,244 | 80,149,268 | TCGTCCTGTGGATAGGAGCTAGGCC |
| TDR | 80,149,294 | 80,149,315 | CCAGGGACTGCTTCAAGTTCAG |
PCR Primers
| Primer | Sequence |
| SU | TGCACTGCAGTCAGGGTTAG |
| SD | GATATCCAGTAAAGTGAAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
