17633 Ino80e Status: ES cell colonies screened / QC no positives
| Gene | Ino80e |
| Chromosome | 7 |
| Deletion Start | 126,861,324 |
| Deletion End | 126,852,717 |
| Deletion Size | 8,608 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,860,772 | 126,860,794 | GGCAGAGGCATAGAGTTTGTTAC |
| TUP | 126,860,742 | 126,860,771 | TGAGGCCCAATCTTTAAGAACAAAGGAGAC |
| TUR | 126,860,718 | 126,860,738 | GTGGACAGCTTTCAGGAGTTG |
| TDF | 126,853,251 | 126,853,271 | GGTCCTGAATCTGGCTGTAAC |
| TDP | 126,853,230 | 126,853,250 | TGCTGGCACAGAGCAACCAGA |
| TDR | 126,853,201 | 126,853,218 | GCTGGGCTCTTCCCTTTC |
PCR Primers
| Primer | Sequence |
| SU | AGGCCTAACTCGGAGAGGAC |
| SD | AGCTCCTTTCTTGGCCTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
