| Gene | Irak3 |
| Chromosome | 10 |
| Deletion Start | 120,201,456 |
| Deletion End | 120,176,236 |
| Deletion Size | 25,221 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,201,188 | 120,201,212 | GGGATTGGGTATGTCAGATGTCAAG |
| TUP | 120,201,161 | 120,201,187 | AAAGCATCCCGTGCTGGGAAGAGATTC |
| TUR | 120,201,136 | 120,201,157 | GCACACTGCAAACCGAGTGAAG |
| TDF | 120,176,497 | 120,176,515 | CACATGGTGGGCCTCACTG |
| TDP | 120,176,469 | 120,176,492 | CTCCTGGGCCTTGCCTCAGACCTT |
| TDR | 120,176,414 | 120,176,436 | TCTCTTCCAAGAGTCAATGTCAC |
PCR Primers
| Primer | Sequence |
| SU | TGCGACGGCGTGTTGCAAG |
| SD | GGAGAGACTTTGATCTTACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17635A-C11 | VGB6 | C57BL/6NTac | |
| 17635A-F2 | VGB6 | C57BL/6NTac | |
| 17635A-G8 | VGB6 | C57BL/6NTac | |
| 17635A-G10 | VGB6 | C57BL/6NTac | |
