17656 Nploc4 Status: ES cell colonies screened / QC no positives
| Gene | Nploc4 |
| Chromosome | 11 |
| Deletion Start | 120,437,510 |
| Deletion End | 120,404,208 |
| Deletion Size | 33,303 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,437,073 | 120,437,093 | CGGGATTGGCTCCTCTTTGAC |
| TUP | 120,437,049 | 120,437,071 | CAGGCGACTTGACGACCACCTGT |
| TUR | 120,437,017 | 120,437,039 | GAAATCGGCCTTCTTTATGACAC |
| TDF | 120,404,684 | 120,404,702 | CAACCTCCTGCCTGCAAAG |
| TDP | 120,404,655 | 120,404,680 | TGTGCGGTTACTCCTGACCTCCTTCA |
| TDR | 120,404,614 | 120,404,634 | GACAGACACTGTTCCACATCC |
PCR Primers
| Primer | Sequence |
| SU | CACAGTCTTCTGAACCCAG |
| SD | GGTTCCTTTTCTGTCTATACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
