17657 Nsdhl Status: ES cell colonies screened / QC no positives
| Gene | Nsdhl |
| Chromosome | X |
| Deletion Start | 72,957,698 |
| Deletion End | 72,926,339 |
| Deletion Size | 31,360 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,926,641 | 72,926,661 | CCTAACAAGCCGCACTAAGAC |
| TUP | 72,926,662 | 72,926,689 | TGGGCACAGACCTATCAGGCTTTATCTG |
| TUR | 72,926,712 | 72,926,732 | CAGCACTGACTGAACAGATGG |
| TDF | 72,957,397 | 72,957,417 | ACCTTGCTTTCCTGCTATCTC |
| TDP | 72,957,419 | 72,957,442 | ACTGGTGATGGTGGTCAGCCCTCT |
| TDR | 72,957,475 | 72,957,493 | GTTCCAGCCAATGCCACTC |
PCR Primers
| Primer | Sequence |
| SU | CACAGGACAGTTTGGCTGG |
| SD | GGCAGACACTGCAACCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
