17658 Nsun6 Status: Cancelled
| Gene | Nsun6 |
| Chromosome | 2 |
| Deletion Start | 15,048,993 |
| Deletion End | 15,013,144 |
| Deletion Size | 35,850 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GGAACTCATAAGGTAGACCGGATTG |
| TUP | | | TCTTGAGTTTACTGTGATCCACCTGCCTC |
| TUR | | | TTCTAGCACTCAGGAGGCAGAG |
| TDF | | | TGGGAGCCATTCTCATTCAAAC |
| TDP | | | TCCACTTTCCTTAACTGGGTCACACTCC |
| TDR | | | TGCTAAAGGACACCAGGATCAG |
PCR Primers
| Primer | Sequence |
| SU | CATGTCAGAGTGGATCTAGG |
| SD | CATAAACACTAACACATTACCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
