| Gene | Pitpnm1 |
| Chromosome | 19 |
| Deletion Start | 4,113,792 |
| Deletion End | 4,101,188 |
| Deletion Size | 12,605 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,101,676 | 4,101,694 | GCATGGATCAGGGCCTCAG |
| TUP | 4,101,706 | 4,101,731 | AACAGACAGGGCAGGCAGGAACTAGA |
| TUR | 4,101,734 | 4,101,752 | GCCAGATGTCCGTCCTCAC |
| TDF | 4,112,820 | 4,112,841 | CCGAGCCAGACCTACATTGTTG |
| TDP | 4,112,843 | 4,112,864 | CCGTGCTGTGCGCAAACTGCAG |
| TDR | 4,112,884 | 4,112,903 | TGTTCCCAGCCTTCCCTTAG |
PCR Primers
| Primer | Sequence |
| SU | GGGTCCCTAAGGTTCTGGTC |
| SD | TAGGTTCCACAGTTCCTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17664A-A12 | VGB6 | C57BL/6NTac | |
| 17664A-H4 | VGB6 | C57BL/6NTac | |
| 17664A-H9 | VGB6 | C57BL/6NTac | |
| 17664A-H11 | VGB6 | C57BL/6NTac | |
