17672 Ptk6 Status: Cancelled
| Gene | Ptk6 |
| Chromosome | 2 |
| Deletion Start | 181,202,501 |
| Deletion End | 181,195,754 |
| Deletion Size | 6,748 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 181,202,469 | 181,202,487 | GGCTCACCTGGGTCCTAAG |
| TUP | 181,202,438 | 181,202,460 | CCTCTGGGACTTCAAGGCACGGA |
| TUR | 181,202,402 | 181,202,421 | GGAGGTCTCCTGCCTGAAAG |
| TDF | 181,196,570 | 181,196,591 | GGTCAGACAGCAATAGGCAATG |
| TDP | 181,196,548 | 181,196,569 | TGGTTGTCCCTGCTCACCAGGA |
| TDR | 181,196,499 | 181,196,518 | TGCCACTTGTACCCTTCTTG |
PCR Primers
| Primer | Sequence |
| SU | AATCTCCTTCCTCTGCTCCC |
| SD | AAGGGAGGAAGAAGCCAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
