17703 Uros Status: Cancelled
| Gene | Uros |
| Chromosome | 7 |
| Deletion Start | 133,702,389 |
| Deletion End | 133,686,940 |
| Deletion Size | 15,450 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 133,701,486 | 133,701,506 | GAAGGCCGGGCTGAATGTAAG |
| TUP | 133,701,466 | 133,701,485 | CCCAGGGCTTCTGCCCACCA |
| TUR | 133,701,407 | 133,701,431 | GTAACACTTTGAGACTTCACTGGAC |
| TDF | 133,687,828 | 133,687,851 | GCTTACTGCTTACGGCTGACTTAC |
| TDP | 133,687,800 | 133,687,823 | AAATGCCACAAAGGAGCCATGCCA |
| TDR | 133,687,762 | 133,687,781 | TCCATTTGGTGGCAGGTGTC |
PCR Primers
| Primer | Sequence |
| SU | GCATAGACACTTGGTTTCAC |
| SD | TTGCACTGAGAACCACAGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
