| Gene | Vstm2a |
| Chromosome | 11 |
| Deletion Start | 16,282,021 |
| Deletion End | 16,258,014 |
| Deletion Size | 24,008 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 16,258,510 | 16,258,530 | GAGGACCCACAAGGAGCTATG |
| TUP | 16,258,532 | 16,258,558 | ATTGCATCATAAGAGGCAGCAGCCTGC |
| TUR | 16,258,567 | 16,258,588 | AGCCTGCAAAGTCCTGAACAAC |
| TDF | 16,281,597 | 16,281,619 | GGATTCGTTCGTTCTCAGAGTTC |
| TDP | 16,281,621 | 16,281,642 | CAGCCGCGGGATGGCATTGTTT |
| TDR | 16,281,652 | 16,281,672 | AGTCCCAAGAAGCTGGTGGTG |
PCR Primers
| Primer | Sequence |
| SU | CATTAGAGGCAAAGTTGGGC |
| SD | GGGTGCTGTATGAGACAGTTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17705A-A3 | VGB6 | C57BL/6NTac | |
| 17705B-A6 | VGB6 | C57BL/6NTac | |
| 17705B-A12 | VGB6 | C57BL/6NTac | |
| 17705B-H6 | VGB6 | C57BL/6NTac | |
