17711 Xiap Status: ES cell colonies screened / QC positives
| Gene | Xiap |
| Chromosome | X |
| Deletion Start | 39,458,257 |
| Deletion End | 39,447,579 |
| Deletion Size | 10,679 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 39,447,719 | 39,447,740 | GCGAGCTGGGTTTCTTTATACC |
| TUP | 39,447,743 | 39,447,768 | TGAAGGAGACACCGTGCAATGTTTCA |
| TUR | 39,447,790 | 39,447,812 | AGCTGAGTCTCCATACTGCCATC |
| TDF | 39,457,514 | 39,457,538 | AGCCCTCACATCGAAGCACTAAATC |
| TDP | 39,457,541 | 39,457,564 | ACCTGTCCCACAGTAGAAGCAGCC |
| TDR | 39,457,582 | 39,457,605 | GTTGAAAGGCATGGGTAATTCCAG |
PCR Primers
| Primer | Sequence |
| SU | TGAGAGAGAAACAGGCTGGG |
| SD | CCAATTAAAGTCATTGGGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17711A-B7 | VGB6 | C57BL/6NTac | |
| 17711A-C5 | VGB6 | C57BL/6NTac | |
| 17711A-G2 | VGB6 | C57BL/6NTac | |
