| Gene | Akt1 |
| Chromosome | 12 |
| Deletion Start | 112,671,558 |
| Deletion End | 112,654,662 |
| Deletion Size | 16,897 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 112,671,511 | 112,671,530 | GGCTGCACAAACGAGGTAAG |
| TUP | 112,671,488 | 112,671,510 | TCCGCACTCCACAGGAGAAGCAG |
| TUR | 112,671,456 | 112,671,478 | CACATTGAAGAGCTAGGCAGACC |
| TDF | 112,655,444 | 112,655,466 | CAGAAGGGAAATGACAACCAGAG |
| TDP | 112,655,420 | 112,655,443 | AACGGAGTTCCCACTGCCTGGAGG |
| TDR | 112,655,369 | 112,655,388 | GGAGAGCAAGCCCAGAGATG |
PCR Primers
| Primer | Sequence |
| SU | ACCTTTGGAGACACGTCAGG |
| SD | TGGACCCTAGAGACAGGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17726A-A11 | VGB6 | C57BL/6NTac | |
| 17726A-C10 | VGB6 | C57BL/6NTac | |
| 17726A-D10 | VGB6 | C57BL/6NTac | |
| 17726A-F11 | VGB6 | C57BL/6NTac | |
| 17726A-F3 | VGB6 | C57BL/6NTac | |
| 17726A-E11 | VGB6 | C57BL/6NTac | |
