| Gene | Lrsam1 |
| Chromosome | 2 |
| Deletion Start | 32,959,585 |
| Deletion End | 32,926,693 |
| Deletion Size | 32,893 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,959,553 | 32,959,572 | GGAAGCGGAAACCCAGTGAG |
| TUP | 32,959,526 | 32,959,551 | AGGCTCGAAAACGCCTGGAGTACCAG |
| TUR | 32,959,504 | 32,959,523 | CGCTGAGCCTTACCAGACAC |
| TDF | 32,927,376 | 32,927,398 | TTAGAACTGTAACCAAGGCACTG |
| TDP | 32,927,350 | 32,927,372 | TCAGGCTCTGAAAGGGACTCCCA |
| TDR | 32,927,294 | 32,927,312 | GCACATGGCCTGAGAAGTC |
PCR Primers
| Primer | Sequence |
| SU | GAAACGCTTAGCACTGGGAG |
| SD | TAGGACGGGATGGCTGATAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17754A-G5 | VGB6 | C57BL/6NTac | |
| 17754A-G10 | VGB6 | C57BL/6NTac | |
| 17754A-A8 | VGB6 | C57BL/6NTac | |
| 17754A-H9 | VGB6 | C57BL/6NTac | |
