| Gene | Ptrf |
| Chromosome | 11 |
| Deletion Start | 100,970,463 |
| Deletion End | 100,958,614 |
| Deletion Size | 11,850 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,970,176 | 100,970,195 | TCCAGGGAGAGCTGAGCAAG |
| TUP | 100,970,151 | 100,970,169 | AAGGCGCACGCCACCACGA |
| TUR | 100,970,088 | 100,970,106 | GCACGGTCTTCACGTTGAC |
| TDF | 100,959,326 | 100,959,350 | CATGTCTGCTCTGTTCTCCTCTTAG |
| TDP | 100,959,301 | 100,959,323 | TGAAGTCAAGCTGCCGGCCAAAC |
| TDR | 100,959,262 | 100,959,281 | GCAGTGCCTCCGACTCTTTC |
PCR Primers
| Primer | Sequence |
| SU | ACTCCCGCTCCAACTATTCC |
| SD | GATCAAGACAACCAGGGCATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17765A-A6 | VGB6 | C57BL/6NTac | |
| 17765A-B10 | VGB6 | C57BL/6NTac | |
| 17765A-D6 | VGB6 | C57BL/6NTac | |
| 17765A-G10 | VGB6 | C57BL/6NTac | |
| 17765A-H3 | VGB6 | C57BL/6NTac | |
