| Gene | Qtrtd1 |
| Chromosome | 16 |
| Deletion Start | 43,881,192 |
| Deletion End | 43,863,002 |
| Deletion Size | 18,191 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,881,088 | 43,881,106 | CATTCCGGGCTGTCTTCTC |
| TUP | 43,881,066 | 43,881,087 | TACACCAGGACTGGCTCTGCCC |
| TUR | 43,881,037 | 43,881,056 | GTTACGCAGCGTCTGATGTG |
| TDF | 43,863,499 | 43,863,517 | TGCACTGGCTCTGGGAATG |
| TDP | 43,863,474 | 43,863,497 | TCTGAGCGGATGAGTGACCACTGT |
| TDR | 43,863,428 | 43,863,449 | GTGCTCTGATAGACACAACCTC |
PCR Primers
| Primer | Sequence |
| SU | CCTAAGTGGAGTGACCTCCC |
| SD | TGAAGACAAAGGTATGGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17811A-B1 | VGB6 | C57BL/6NTac | |
| 17811A-E6 | VGB6 | C57BL/6NTac | |
| 17811A-D5 | VGB6 | C57BL/6NTac | |
| 17811A-A4 | VGB6 | C57BL/6NTac | |
| 17811A-H7 | VGB6 | C57BL/6NTac | |
| 17811A-E7 | VGB6 | C57BL/6NTac | |
