17815 Rhot1 Status: Cancelled
| Gene | Rhot1 |
| Chromosome | 11 |
| Deletion Start | 80,242,678 |
| Deletion End | 80,209,251 |
| Deletion Size | 33,428 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,209,552 | 80,209,572 | TCGCCTGTCAGTCACTCATTG |
| TUP | 80,209,573 | 80,209,597 | CCAGGCAGATGTTGGAACAGGCAGA |
| TUR | 80,209,598 | 80,209,616 | CGCAGTCCCAAGTGCTCAG |
| TDF | 80,241,696 | 80,241,720 | CAGGCTCCTACCTGTCTTAAAGAAG |
| TDP | 80,241,723 | 80,241,750 | AAAGAGTGAGGCCTGACAAGCAGAGTTG |
| TDR | 80,241,755 | 80,241,773 | TGGTGCTTGCGTGGAAGTC |
PCR Primers
| Primer | Sequence |
| SU | CATCCTCTGGCAGTGTTCAG |
| SD | CTCATATTTACGTCTCTCAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
