| Gene | Sdsl |
| Chromosome | 5 |
| Deletion Start | 120,463,202 |
| Deletion End | 120,458,277 |
| Deletion Size | 4,926 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,462,347 | 120,462,366 | GCTCTTGCTCTTGCTTCTCC |
| TUP | 120,462,316 | 120,462,340 | CTCTCCTCTCGTCCTGGTCCCACAG |
| TUR | 120,462,267 | 120,462,284 | GAACGCAACCTCGGTGAC |
| TDF | 120,459,256 | 120,459,274 | TGCCCTTCCCACATTCCTG |
| TDP | 120,459,234 | 120,459,255 | TCTCCACATGTCCGTCCTGGCC |
| TDR | 120,459,181 | 120,459,203 | GGAAGCACCATAACGGATAACTC |
PCR Primers
| Primer | Sequence |
| SU | GGTTACACTCTGTGTCCTC |
| SD | ATACTGTGGCAGGGTCTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17818A-B8 | VGB6 | C57BL/6NTac | |
| 17818A-F4 | VGB6 | C57BL/6NTac | |
| 17818A-G2 | VGB6 | C57BL/6NTac | |
