| Gene | Mef2d |
| Chromosome | 3 |
| Deletion Start | 88,168,705 |
| Deletion End | 88,155,812 |
| Deletion Size | 12,894 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,156,360 | 88,156,378 | GGCCTACGAGCTGAGTGTG |
| TUP | 88,156,382 | 88,156,401 | TGCGACTGCGAGATCGCGCT |
| TUR | 88,156,402 | 88,156,425 | TTGTTGGAGTGGTTGAAGATGATG |
| TDF | 88,167,954 | 88,167,976 | CCCACTCTCATTCTGTTGTGTTG |
| TDP | 88,167,978 | 88,168,003 | CCCTGTCTGGTGACTTCTTCCCACTC |
| TDR | 88,168,027 | 88,168,045 | TGCCAGGGCTGGAGTTTAG |
PCR Primers
| Primer | Sequence |
| SU | CCTCTATAGCCAATATTGTTGC |
| SD | TCTATCTGGGAATGGGATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17833A-B4 | VGB6 | C57BL/6NTac | |
| 17833A-B5 | VGB6 | C57BL/6NTac | |
| 17833A-C8 | VGB6 | C57BL/6NTac | |
| 17833A-G3 | VGB6 | C57BL/6NTac | |
| 17833A-G11 | VGB6 | C57BL/6NTac | |
| 17833A-F8 | VGB6 | C57BL/6NTac | |
