17837 Ribc1 Status: ES cell colonies screened / QC no positives
| Gene | Ribc1 |
| Chromosome | X |
| Deletion Start | 152,009,638 |
| Deletion End | 152,004,634 |
| Deletion Size | 5,005 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 152,009,583 | 152,009,603 | GGCAGCCATTGAAGCTAGAAG |
| TUP | 152,009,555 | 152,009,578 | CGTGAAAAGGAACGCCAAAGCCGA |
| TUR | 152,009,532 | 152,009,552 | ACTCGGTTCCGCACATTGAAG |
| TDF | 152,005,263 | 152,005,281 | ACAGCAGCGGCAACAGGAG |
| TDP | 152,005,229 | 152,005,257 | CCTCACAGAGGTCAAGAAACAAGTCACCA |
| TDR | 152,005,191 | 152,005,212 | TCTTTGGGCAACTTGAGGATTC |
PCR Primers
| Primer | Sequence |
| SU | TAGTGAGACTTGCTTTCAGAG |
| SD | CGCTGAATATGCTCATCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
