| Gene | Smc1b |
| Chromosome | 15 |
| Deletion Start | 85,131,934 |
| Deletion End | 85,120,622 |
| Deletion Size | 11,313 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,131,844 | 85,131,866 | CAAGAGGTTCACCTGCATCATTG |
| TUP | 85,131,819 | 85,131,840 | CCAACGGCTCCGGTAACCAGCG |
| TUR | 85,131,797 | 85,131,815 | CCGAGGCCGTGGATTTCTG |
| TDF | 85,120,740 | 85,120,760 | AGCTGGATCGTTACAAACTGC |
| TDP | 85,120,718 | 85,120,739 | TTAAGGAGCAAGTCAGACGGAA |
| TDR | 85,120,663 | 85,120,683 | TTCTGCCTTCTGTTCCCACTG |
PCR Primers
| Primer | Sequence |
| SU | GAAATAAGCAAAGCTACAGCG |
| SD | TTCCTGAACACATATGAGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17839B-C3 | VGB6 | C57BL/6NTac | |
| 17839B-D6 | VGB6 | C57BL/6NTac | |
| 17839B-D10 | VGB6 | C57BL/6NTac | |
