17845 Ap3s2 Status: Cancelled
| Gene | Ap3s2 |
| Chromosome | 7 |
| Deletion Start | 79,920,574 |
| Deletion End | 79,915,152 |
| Deletion Size | 5,423 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,919,993 | 79,920,013 | GCACACCTCAAAGCTCAGAAC |
| TUP | 79,919,958 | 79,919,985 | ATGGAGGCGAGAGGATCAGGACTTCAAA |
| TUR | 79,919,914 | 79,919,935 | GTAGCTCAAGCTGGCTTAGAAC |
| TDF | 79,915,267 | 79,915,289 | TGGGTTCTCAACTGCTACTACTG |
| TDP | 79,915,240 | 79,915,266 | TCTTCCAGTTTGATTGGTGGCTCTGAC |
| TDR | 79,915,213 | 79,915,233 | GGCATAGTGCCGGTAAATCAG |
PCR Primers
| Primer | Sequence |
| SU | TACTGGTCCCTCAAACTGGC |
| SD | ACTCATGGATGTCAATGAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
