| Gene | Capn8 |
| Chromosome | 1 |
| Deletion Start | 182,599,622 |
| Deletion End | 182,565,064 |
| Deletion Size | 34,559 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 182,565,216 | 182,565,234 | GGCATGTCCGTCAGCTTTG |
| TUP | 182,565,237 | 182,565,263 | CTACAGGGATCTTGGACCAGGCTCTGC |
| TUR | 182,565,298 | 182,565,320 | TGGTCACTTCCCTATTGCCTTAC |
| TDF | 182,598,656 | 182,598,678 | CAACAGGCTCAATGGTTCGTATG |
| TDP | 182,598,679 | 182,598,703 | AGGCTCTTGCTGGAGGCTCCACAAT |
| TDR | 182,598,747 | 182,598,765 | CCGGAGGCTTCCTCAAGTC |
PCR Primers
| Primer | Sequence |
| SU | GAGAAATCACCCCAGCCTATC |
| SD | GTATACAGAATCTAACTGACAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17847A-H10 | VGB6 | C57BL/6NTac | |
| 17847A-D10 | VGB6 | C57BL/6NTac | |
| 17847A-C7 | VGB6 | C57BL/6NTac | |
