| Gene | Cnot4 |
| Chromosome | 6 |
| Deletion Start | 35,080,131 |
| Deletion End | 35,046,197 |
| Deletion Size | 33,935 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,079,250 | 35,079,273 | AAACCCTCTGGCTAAATGTGATTC |
| TUP | 35,079,213 | 35,079,242 | ACAAATGAGAAGGCTAGGTCAGAATGGTCA |
| TUR | 35,079,178 | 35,079,199 | GCTTTCAATTCCAGCCAACCTG |
| TDF | 35,046,307 | 35,046,325 | TGTCCGCCTCTTCCCATTC |
| TDP | 35,046,275 | 35,046,302 | CAACAAGGACAGCAGCCTACAAGTCTCC |
| TDR | 35,046,231 | 35,046,252 | TCCTCTCCTTTCCTCTCAGTTC |
PCR Primers
| Primer | Sequence |
| SU | CTGAAAGCTGAAGCTGCATC |
| SD | AGGAACAGGTCTAGCTAATCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17848A-D11 | VGB6 | C57BL/6NTac | |
| 17848A-D4 | VGB6 | C57BL/6NTac | |
