| Gene | Rest |
| Chromosome | 5 |
| Deletion Start | 77,283,054 |
| Deletion End | 77,267,944 |
| Deletion Size | 15,111 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 77,267,981 | 77,268,003 | GTCTCTTCAACAACAGTGCCAAC |
| TUP | 77,268,005 | 77,268,028 | TGGGCATGGCCTTAACCAACGACA |
| TUR | 77,268,051 | 77,268,069 | GGCTGCCAGTTCAGCTTTC |
| TDF | 77,282,501 | 77,282,518 | TGGCTCCTCTGGGCTTTG |
| TDP | 77,282,519 | 77,282,543 | TGACGTGGACACTGAGCAGAAGACA |
| TDR | 77,282,558 | 77,282,577 | GCTCTGCTGCGGAGTCTTTC |
PCR Primers
| Primer | Sequence |
| SU | CAATGGGTCAAAGAATTCGC |
| SD | GCAAGTTAGGAAGCAATTACCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17856A-B2 | VGB6 | C57BL/6NTac | |
| 17856A-D6 | VGB6 | C57BL/6NTac | |
| 17856A-E12 | VGB6 | C57BL/6NTac | |
