17862 Alad Status: Cancelled
| Gene | Alad |
| Chromosome | 4 |
| Deletion Start | 62,514,183 |
| Deletion End | 62,509,231 |
| Deletion Size | 4,953 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,513,583 | 62,513,602 | GCCCTCCCTTGCTCTTACAG |
| TUP | 62,513,552 | 62,513,579 | TGTTCCTGATGATGTCCAGCCTATCGCC |
| TUR | 62,513,511 | 62,513,531 | GACAGTCACCACACCTCCTAC |
| TDF | 62,509,467 | 62,509,486 | GCCCACGACAAGAGAATGAC |
| TDP | 62,509,438 | 62,509,461 | CAGTGTCTGGAGCTGGCTACAGCC |
| TDR | 62,509,389 | 62,509,408 | GACCACCAGGGAGAGGTATG |
PCR Primers
| Primer | Sequence |
| SU | GTGAACCATGGCCCAAATAC |
| SD | AGGTTTCAAGCTCCAACTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
