| Gene | Alkbh3 |
| Chromosome | 2 |
| Deletion Start | 94,008,518 |
| Deletion End | 93,996,188 |
| Deletion Size | 12,331 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,008,492 | 94,008,512 | AAGAGACAGCGAGCCCGAGTG |
| TUP | 94,008,468 | 94,008,491 | CAGGGAGGCTGGGCTACACCTACC |
| TUR | 94,008,432 | 94,008,454 | CAGGTTCTTGCCTGGCTGAGTAG |
| TDF | 93,996,395 | 93,996,414 | CTGTCCCTGTGCTTGCTGTC |
| TDP | 93,996,370 | 93,996,392 | TGGCTTCCTGTGCTGTGGACTCT |
| TDR | 93,996,336 | 93,996,356 | GGTGTGGCTGGTGTTCTCTTC |
PCR Primers
| Primer | Sequence |
| SU | ATGTGGTCTGCTCTTGGGAC |
| SD | CAGCCACTTAAATGCTTTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17884A-H2 | VGB6 | C57BL/6NTac | |
| 17884A-A9 | VGB6 | C57BL/6NTac | |
| 17884A-B3 | VGB6 | C57BL/6NTac | |
| 17884A-G4 | VGB6 | C57BL/6NTac | |
| 17884A-F7 | VGB6 | C57BL/6NTac | |
| 17884A-A7 | VGB6 | C57BL/6NTac | |
