17890 Gast Status: ES cell colonies screened / QC no positives
| Gene | Gast |
| Chromosome | 11 |
| Deletion Start | 100,336,903 |
| Deletion End | 100,336,486 |
| Deletion Size | 418 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,336,583 | 100,336,602 | CTGGACCAGGGACCAATGAG |
| TUP | 100,336,604 | 100,336,627 | ACCTGGAACAGCGCCAGTTCAACA |
| TUR | 100,336,641 | 100,336,661 | AGCTGCCTTCGATGGTGAGAG |
| TDF | 100,336,764 | 100,336,786 | GGCCTTCTTCTCTCCGTTCTTAC |
| TDP | 100,336,787 | 100,336,813 | CTCCTGTTACCTCCTCAGACCTGTCCA |
| TDR | 100,336,829 | 100,336,851 | GGCCTCTTCTTCTTCCTCCATTC |
PCR Primers
| Primer | Sequence |
| SU | AAGAGTCACCGGACCTAGCC |
| SD | CCACCCAGGTAGATCCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
