| Gene | Gm5113 |
| Chromosome | 7 |
| Deletion Start | 30,178,932 |
| Deletion End | 30,178,492 |
| Deletion Size | 441 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,178,525 | 30,178,543 | GCAGCTCAGGCCACATCAG |
| TUP | 30,178,547 | 30,178,569 | TCAGAGCGTCCACACTGGTGACA |
| TUR | 30,178,574 | 30,178,595 | TCCACAGTCCTTACATGCCAAG |
| TDF | 30,178,800 | 30,178,821 | CCCTATGAGTGTGGAGAGTGTG |
| TDP | 30,178,824 | 30,178,849 | CAGGCCTTTATCCGTGGCTCTCAGCT |
| TDR | 30,178,854 | 30,178,875 | CGGAATGAACTCGGTAGTGGTC |
PCR Primers
| Primer | Sequence |
| SU | ATTCTGCATCCAAGAACTCAC |
| SD | TGGCCATCTATGTGGGATTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17891A-A1 | VGB6 | C57BL/6NTac | |
| 17891A-B8 | VGB6 | C57BL/6NTac | |
| 17891A-B6 | VGB6 | C57BL/6NTac | |
| 17891A-H2 | VGB6 | C57BL/6NTac | |
| 17891A-D4 | VGB6 | C57BL/6NTac | |
