17893 Mphosph9 Status: ES cell colonies screened / QC no positives
| Gene | Mphosph9 |
| Chromosome | 5 |
| Deletion Start | 124,324,977 |
| Deletion End | 124,297,559 |
| Deletion Size | 27,419 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,324,760 | 124,324,782 | TGCTGGTTTGCCTTAAAGAACAG |
| TUP | 124,324,735 | 124,324,758 | ACAGCCAACCTCTGTGTGGGTCCA |
| TUR | 124,324,696 | 124,324,717 | CAGCAGAGAGACAGAGGGATAC |
| TDF | 124,297,725 | 124,297,746 | CACGGTCCTGAGAGCATCTTTC |
| TDP | 124,297,693 | 124,297,718 | ACTGACAGGATCAGGCAGAACCTCAA |
| TDR | 124,297,605 | 124,297,625 | GTCTTTGGCCTCCAGTTTCTG |
PCR Primers
| Primer | Sequence |
| SU | CCCTACCTAGTATTGCTGTTC |
| SD | AAGGCATGGCTTTGTGTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 142 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
