| Gene | Rgs13 |
| Chromosome | 1 |
| Deletion Start | 144,171,478 |
| Deletion End | 144,139,420 |
| Deletion Size | 32,059 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 144,170,815 | 144,170,833 | CCTTACAGGCCAGGCCATC |
| TUP | 144,170,787 | 144,170,814 | CCCAGCCTTGTGATCTTACTGCCTAAGC |
| TUR | 144,170,723 | 144,170,744 | CCAGGTCACAGGGAAATTGGAG |
| TDF | 144,139,941 | 144,139,962 | CAGACTACCACTAGCAAACCAC |
| TDP | 144,139,919 | 144,139,940 | TGGCATGTCCCAAGGAAGCAGA |
| TDR | 144,139,863 | 144,139,885 | AGCACCCAGGGAAAGAATGAAAG |
PCR Primers
| Primer | Sequence |
| SU | ATACAGCTCTCAGAGTTCTAC |
| SD | AATTCAAGATATTCTGAGCATATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 111 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17910A-C11 | VGB6 | C57BL/6NTac | |
| 17910A-E2 | VGB6 | C57BL/6NTac | |
