17911   Snip1     Status: ES cell colonies screened / QC positives

GeneSnip1
Chromosome4
Deletion Start125,072,976
Deletion End125,066,756
Deletion Size6,221
Genome Build38
MGI IDMGI:2156003
NCBI Gene ID76793
BAC ID109H13
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 4

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF125,067,094125,067,115CCTCGTCTGCCTACAAAGACAC
TUP125,067,118125,067,141TGGGTCTTGGCGACTTCTTCGCAG
TUR125,067,144125,067,162GGAGGCATTGGCGAATGAG
TDF125,072,168125,072,192TTGGGAATACATCTTTACCGTTTGG
TDP125,072,199125,072,222CCCTGTGAGGAAAGCAGGGCCAAT
TDR125,072,238125,072,256AGCCGTGGTTTCTCACCTG

PCR Primers

PrimerSequence
SUCCTGCCTTTTCAGTACTCC
SDTGGTCCACAAAGCTCAACAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR69 bp++-
TDFTDR89 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17911A-A10VGB6C57BL/6NTac
17911A-A12VGB6C57BL/6NTac
17911A-C12VGB6C57BL/6NTac
17911A-D3VGB6C57BL/6NTac
17911A-E5VGB6C57BL/6NTac
17911A-H9VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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