17912 Usp12 Status: Cancelled
| Gene | Usp12 |
| Chromosome | 5 |
| Deletion Start | 146,794,659 |
| Deletion End | 146,763,203 |
| Deletion Size | 31,457 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 146,794,134 | 146,794,152 | GGCAGGAAGGAAGGGTCTC |
| TUP | 146,794,106 | 146,794,131 | TCCTACTGGAAGACGGAAGTCCTGGC |
| TUR | 146,794,083 | 146,794,102 | CACCTAACCCGAGGAGGAAC |
| TDF | 146,763,847 | 146,763,865 | TGCAGCCTCTCAGATCCTC |
| TDP | 146,763,823 | 146,763,846 | CATGCTAACGGCCCAGCCAGCTTT |
| TDR | 146,763,799 | 146,763,822 | GCACTACATACCAAGCACAAACAG |
PCR Primers
| Primer | Sequence |
| SU | GTGTGCTGTGAGCGGCTTC |
| SD | CTACAGTAGTAGCCACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
