17913 Cog1 Status: ES cell colonies screened / QC no positives
| Gene | Cog1 |
| Chromosome | 11 |
| Deletion Start | 113,662,396 |
| Deletion End | 113,649,564 |
| Deletion Size | 12,833 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,650,323 | 113,650,345 | ATCCTGATGTCCTCCCATGAAAC |
| TUP | 113,650,349 | 113,650,375 | TAAGACCCGATGCGGATCGAATTCTGA |
| TUR | 113,650,389 | 113,650,409 | CGCCCTCTGCTCATTCCTAAG |
| TDF | 113,662,218 | 113,662,239 | GGCACTCTCCAGAGTAGGTGAC |
| TDP | 113,662,241 | 113,662,263 | CGACGACGCATCCTGGCTCCTTA |
| TDR | 113,662,285 | 113,662,304 | GGTGCAGGTGAGTCGTCTTC |
PCR Primers
| Primer | Sequence |
| SU | TTCTGTCGCACGTACTCCAC |
| SD | CTCCCTTGGAACAGAACAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
