| Gene | Rab27a |
| Chromosome | 9 |
| Deletion Start | 73,095,507 |
| Deletion End | 73,075,418 |
| Deletion Size | 20,090 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,075,657 | 73,075,681 | ACACAAGAAAGTGGCTAGGTACAAC |
| TUP | 73,075,687 | 73,075,711 | TGCCTTTAATCCCAGCATGGAGGCA |
| TUR | 73,075,726 | 73,075,746 | GACCTGGCTGCTCTCAAACTC |
| TDF | 73,094,775 | 73,094,793 | TGGCAGCTACTGGTGGTTG |
| TDP | 73,094,794 | 73,094,818 | CCTAGACGACACTCCTAGCCCACCC |
| TDR | 73,094,853 | 73,094,874 | TCATCCTGAGGCAGACTGTATG |
PCR Primers
| Primer | Sequence |
| SU | CCAACAAAGTATGCAAACAAATAC |
| SD | CTGGATTCTCAAGACCGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17948A-C5 | VGB6 | C57BL/6NTac | |
| 17948A-H3 | VGB6 | C57BL/6NTac | |
| 17948A-F9 | VGB6 | C57BL/6NTac | |
| 17948A-A1 | VGB6 | C57BL/6NTac | |
