| Gene | Gsta3 |
| Chromosome | 1 |
| Deletion Start | 21,264,987 |
| Deletion End | 21,249,775 |
| Deletion Size | 15,213 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,250,673 | 21,250,692 | CTTGCTGCACTAGGAAGTCC |
| TUP | 21,250,698 | 21,250,721 | TGACGCCCTGGATCAATTGCTGTC |
| TUR | 21,250,751 | 21,250,770 | GAGCCTCAGGCAAACAGTTG |
| TDF | 21,264,382 | 21,264,403 | TCTGTGGTGCTTGTGAGTAAAC |
| TDP | 21,264,405 | 21,264,429 | TTGCATCTACTGGGCAAGGGCTGTA |
| TDR | 21,264,438 | 21,264,459 | GGATCAGAGGTTGGCAGTGTAG |
PCR Primers
| Primer | Sequence |
| SU | TCCCACACATTATCATCCTCC |
| SD | TATTTGGTCAGGAAGGGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17960A-A10 | VGB6 | C57BL/6NTac | |
| 17960A-B6 | VGB6 | C57BL/6NTac | |
| 17960A-H1 | VGB6 | C57BL/6NTac | |
