17968 Angel2 Status: ES cell colonies screened / QC positives
| Gene | Angel2 |
| Chromosome | 1 |
| Deletion Start | 190,945,183 |
| Deletion End | 190,928,703 |
| Deletion Size | 16,481 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 190,929,438 | 190,929,461 | TGGGAAATAGCACACTGTCGTTAC |
| TUP | 190,929,464 | 190,929,486 | AAGCCGTTGGGATCTGCCCACTC |
| TUR | 190,929,487 | 190,929,509 | CCATAGCACAGGAACCAGGAATG |
| TDF | 190,945,024 | 190,945,043 | GCCTTCCCAATGAGCACAAC |
| TDP | 190,945,046 | 190,945,070 | CTCAGATCACCTGCCTTTGCTGGCC |
| TDR | 190,945,090 | 190,945,109 | CCCGTGAATGAGCAGCAGTC |
PCR Primers
| Primer | Sequence |
| SU | GGTCTCACGCAGCTCCTG |
| SD | GGGCCAATGTTCTCTACAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
