| Gene | D11Wsu99e |
| Chromosome | 11 |
| Deletion Start | 113,683,881 |
| Deletion End | 113,663,300 |
| Deletion Size | 20,582 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,683,583 | 113,683,602 | CCGCTTCGGAATGAGGTTTG |
| TUP | 113,683,563 | 113,683,582 | TTCGGCCCGACGAGACGGAC |
| TUR | 113,683,537 | 113,683,559 | CGCCTCAATTTAGGAAGGACCAC |
| TDF | 113,663,794 | 113,663,816 | CACTCCTGGGCATGTACTCAAAG |
| TDP | 113,663,761 | 113,663,786 | TGTCCCACCACAGAGACACTTACACA |
| TDR | 113,663,714 | 113,663,737 | GAACTCCACTTCCTGGCTACTATG |
PCR Primers
| Primer | Sequence |
| SU | AAGTTCCCGGTCAGTCTTCC |
| SD | ACGACGTAGATCTGATGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17971A-A7 | VGB6 | C57BL/6NTac | |
| 17971A-B9 | VGB6 | C57BL/6NTac | |
