17972 Eed Status: ES cell colonies screened / QC no positives
| Gene | Eed |
| Chromosome | 7 |
| Deletion Start | 89,980,522 |
| Deletion End | 89,954,845 |
| Deletion Size | 25,678 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,980,455 | 89,980,476 | CGGCCAAGAAGCAGAAGTTGAG |
| TUP | 89,980,431 | 89,980,453 | AGCGACGAGAACAGCAACCCGGA |
| TUR | 89,980,344 | 89,980,364 | CGACCGCAGTTACCAAAGAAC |
| TDF | 89,955,686 | 89,955,706 | GCTGGAGCTTGGGCTTCTAAG |
| TDP | 89,955,663 | 89,955,685 | CAAGGAAGCGCCCTGGGTTTCCT |
| TDR | 89,955,639 | 89,955,661 | GAGCACCAGGCTCTAAGGTATTC |
PCR Primers
| Primer | Sequence |
| SU | GGTGCAAAGTGTCGAGAGG |
| SD | ACGCTACATGGTGGCAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 133 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
