17983 Ikbkb Status: Cancelled
| Gene | Ikbkb |
| Chromosome | 8 |
| Deletion Start | 22,706,183 |
| Deletion End | 22,678,794 |
| Deletion Size | 27,390 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GGAAGGTGGGTGAGCTTTC |
| TUP | | | TTGGCGCCTCTTCTGACCTGGC |
| TUR | | | CATTGGACAACGCCAAGGTTAG |
| TDF | | | AGCTGAGCCTGCTTGGGTTC |
| TDP | | | TCTCCTCACACCCAAGTGGGCCT |
| TDR | | | AAGGGCCACCAGCTCATAGAG |
PCR Primers
| Primer | Sequence |
| SU | ATGTCCTCAGCGGGTGTC |
| SD | TCTGTAGTGTACTGACTCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
