17992 Nlgn2 Status: Cancelled
| Gene | Nlgn2 |
| Chromosome | 11 |
| Deletion Start | 69,834,435 |
| Deletion End | 69,825,106 |
| Deletion Size | 9,330 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,833,920 | 69,833,938 | CACAAACGCACACGCAGAC |
| TUP | 69,833,895 | 69,833,919 | CCTCAAGCACAGGACCGACTTCTGC |
| TUR | 69,833,842 | 69,833,860 | GAGGCTTGCAGGGACATAC |
| TDF | 69,825,673 | 69,825,692 | CACGGAGCTAAGCGTGACTG |
| TDP | 69,825,653 | 69,825,672 | TGGCAGTGGGTGCCTCCCTC |
| TDR | 69,825,628 | 69,825,652 | CAAAGGCAAGGATGTTGAGGAAGAG |
PCR Primers
| Primer | Sequence |
| SU | ATGGAGAGAAATAGACTCCTC |
| SD | CGCAGAAATTCTTGGAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
