17996 Qars Status: ES cell colonies screened / QC positives
| Gene | Qars |
| Chromosome | 9 |
| Deletion Start | 108,418,269 |
| Deletion End | 108,410,417 |
| Deletion Size | 7,853 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,410,966 | 108,410,984 | CAGGCACTGAGGCATGGAG |
| TUP | 108,410,986 | 108,411,011 | TAGAGACCTCTGGTGTGGATTGCAGA |
| TUR | 108,411,012 | 108,411,033 | GCAGGCTTCTCATTGTGGTAAC |
| TDF | 108,417,289 | 108,417,310 | TGGCTACAGATTCCAGCACAAG |
| TDP | 108,417,314 | 108,417,336 | CCCGAAGACCCTGTTGAAGTGCC |
| TDR | 108,417,351 | 108,417,369 | CCTACCGGGTTCAGGTCAC |
PCR Primers
| Primer | Sequence |
| SU | GGATAGTGTCGGGTTCCTTG |
| SD | AACTGGAAAGGCCACAACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17996A-B4 | VGB6 | C57BL/6NTac | |
| 17996A-G3 | VGB6 | C57BL/6NTac | |
