18003 Abcd1 Status: Cancelled
| Gene | Abcd1 |
| Chromosome | X |
| Deletion Start | 73,737,567 |
| Deletion End | 73,717,034 |
| Deletion Size | 20,534 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,717,551 | 73,717,574 | ACTTCTCCCAACAGACTTACTACC |
| TUP | 73,717,582 | 73,717,608 | CAACATGGATGGACGACTTCGAAACCC |
| TUR | 73,717,628 | 73,717,647 | GCAGCAAAGGCTACCACATC |
| TDF | 73,737,191 | 73,737,210 | ATCGCACTGCTCTCCATCAC |
| TDP | 73,737,212 | 73,737,236 | CATCGACCCTCCCTATGGTAGGTGC |
| TDR | 73,737,238 | 73,737,259 | CAGCCTCACCCACAATTCCAAG |
PCR Primers
| Primer | Sequence |
| SU | TAGACGCTGTCGTTACTGCC |
| SD | GCAGGGATGTAACCCACAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
